rabbit anti lkb1  (Bioss)

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: Sequences of primers used for semi-quantitative PCR.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Sequencing

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: Bioinformatics analysis. (A) Volcano plots (log 2 -fold change) were used to show the upregulated and downregulated genes in patients with severe NAFLD compared with normal and mild patients. (B) GO enriched terms associated with the DEGs in patients with NAFLD. (C) KEGG enriched pathways related to DEGs in patients with NAFLD. (D) Relative expression of LKB1 in different groups. Data are presented as the mean ± SD. *** P<0.001 vs. Control; ## P<0.01 and ### P<0.001 vs. FFA group. NAFLD, non-alcoholic fatty liver disease; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; DEG, differentially expressed gene; LKB1, liver kinase B1; FFA, free fatty acid; sh-, short hairpin.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Expressing, Control

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: Effects of overexpression and knockdown of Salusin-α on downstream molecules. (A) LKB1, AMPK, SREBP-1c, ACC and FASN PCR electrophoresis results. The first column shows the molecular changes of each group after transfecting virus for 24 h. The second column shows the molecular changes after 24 h of virus transfection followed by 24 h of FFA induction. (B-F) Relative expression of (B) LKB1, (C) AMPK, (D) SREBP-1c, (E) ACC and (F) FASN in the different groups. Data are presented as the mean ± SD. * P<0.05, ** P<0.01 and *** P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. FFA group. LKB1, liver kinase B1; AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; SREBP-1c, sterol regulatory element binding protein-1c; ACC, acetyl-CoA carboxylase; FASN, fatty acid synthase; FFA, free fatty acid; sh-, short hairpin; ns, not significant.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Over Expression, Knockdown, Electrophoresis, Virus, Transfection, Expressing, Control, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: Validation of Salusin-α regulation of lipid metabolism via the LKB1/AMPK pathway by western blotting. (A) After lentiviral transfection of HepG2 cells for 24 h, western blotting was used to detect the expression of several proteins in the LKB1/AMPK downstream pathway. Expression of (B) p-LKB1/LKB1, (C) p-AMPK/AMPK, (D) SREBP-1c and (E) FASN in each group. (F) Lentiviruses were transfected for 24 h and then cells were induced with FFA for 24 h. The bar graphs show the densitometric analysis of the western blots. (G-J) Expression of (G) p-LKB1/LKB1, (H) p-AMPK/AMPK, (I) SREBP-1c and (J) FASN in the different treatment groups. Data are presented as the mean ± SD. * P<0.05, ** P<0.01 and *** P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. FFA group. LKB1, liver kinase B1; AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; SREBP-1c, sterol regulatory element binding protein-1c; FASN, fatty acid synthase; p-, phosphorylated; FFA, free fatty acid; sh-, short hairpin; ns, not significant.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Western Blot, Transfection, Expressing, Control, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: PCR was used to confirm the molecular changes following treatment with the inhibitors. (A) Images of agarose gels following PCR amplification of LKB1, AMPK, SREBP-1c, ACC, FASN and GAPDH in each group. (B-F) Relative expression of (B) LKB1, (C) AMPK, (D) SREBP-1c, (E) ACC and (F) FASN. Data are presented as the mean ± SD. * P<0.05, ** P<0.01 and *** P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. FFA group. LKB1, liver kinase B1; AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; SREBP-1c, sterol regulatory element binding protein-1c; ACC, acetyl-CoA carboxylase; FASN, fatty acid synthase; FFA, free fatty acid; sh, short hairpin; ns, not significant.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Amplification, Expressing, Control, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: Western blotting was used to confirm the changes in the expression of downstream molecules following addition of inhibitors. (A) The induction of FFA in HepG2 cells after 24 h transfection with the lentivirus and Compound C was compared with that after transfection with the virus alone, and western blotting was used to detect the expression of each protein downstream in the LKB1/AMPK pathway. (B-E) Protein expression levels of (B) p-LKB1/LKB1, (C) p-AMPK/AMPK, (D) SREBP-1c and (E) FASN in each group. Data are presented as the mean ± SD. * P<0.05, ** P<0.01 and *** P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. FFA group. FFA, free fatty acid; LKB1, liver kinase B1; AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; p-, phosphorylated; SREBP-1c, sterol regulatory element binding protein-1c; FASN, fatty acid synthase; sh, short hairpin; ns, not significant.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Western Blot, Expressing, Transfection, Virus, Control, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Salusin-α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

doi: 10.3892/ijmm.2024.5397

Figure Lengend Snippet: Hypothesized molecular mechanism depicting how Salusin-α ameliorates lipid metabolism disorders via regulation of the LKB1/AMPK signaling pathway. Salusinα was overexpresses or knocked down using lentiviruses. Salusin-α upregulated LKB1 expression and thus activated AMPK, which resulted in a decrease in the downstream SREBP-1c/FASN/ACC levels, thus reducing the severity of NAFLD. AS and NAFLD are causative factors in the deterioration of the other respective disease, and the elevated levels of LDLs in blood vessels leads to an increase in fatty acid content and worsens the degree of NAFLD. Gray arrows indicate the mechanistic process by which lentiviral vectors transport and regulate Salusin-α expression. Red arrows indicate changes in the expression of signaling pathway molecules following overexpression of Salusin-α in HepG2 cells, and green arrows indicate the changes in intravascular lipoprotein during the development of AS. LKB1, liver kinase B1; AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; NAFLD, non-alcoholic fatty liver disease; FASN, fatty acid synthase; ACC, acetyl-CoA carboxylase; AS, atherosclerosis; LDL, low density lipoprotein; VLDL, very low-density lipoprotein; HDL, high-density lipoprotein; ROS, reactive oxygen species; SREBP-1c, sterol regulatory element binding protein-1c; ER, endoplasmic reticulum; p-, phosphorylated.

Article Snippet: A2122; ABclonal Technology), anti-p-LKB1(Ser 428) rabbit polyclonal antibodies (1:1,000; cat. no. AP0602; ABclonal Biotech Co., Ltd.), antiSREBP1 rabbit polyclonal antibodies (1:1,000; cat. no. ab28481, Abcam), anti-FASN rabbit polyclonal antibodies (1:1,000; cat. no. FNab03019; Wuhan Fine Biotech Co., Ltd.), or anti-GAPDH rabbit monoclonal antibodies (1:10,000; cat. no. ab181602; Abcam) at 4°C overnight.

Techniques: Expressing, Over Expression, Binding Assay